Five cancer cell lines including non-small cell lung carcinoma (H1299), osteosarcoma (U-2 OS and Saos-2), breast cancer (MDA-MB-468) and glioblastoma (U-87 MG) were used to evaluate the constructs and all lines were sensitive to the full-length and truncated forms of ING1. This evidence concerns the gene ING1 and osteosarcoma.