To investigate the effect of the H1047R mutation on cell morphology and actin cytoskeleton structure, we used cell lines harboring either WT or mutant (MUT; H1047R) p110α of PI3K, which were generated by asymmetric deletion of the PIK3CA allele from the CRC parental cell line HCT116. The gene discussed is PIK3CA; the disease is colorectal carcinoma.