GSK3β is the central component in the multiprotein destruction complex consisting of GSK3β/Axin/APC/casein kinase 1 (CK1) that controls the phosphorylation and degradation of β-catenin, and functions as a negative regulator of Wnt signaling pathway.30–32 Therefore, we further investigated whether GSK3β is involved in Z86-promoted β-catenin phosphorylation and degradation in colon cancer cell lines. The gene discussed is AXIN1; the disease is malignant colon neoplasm.