In order to determine whether the expression of MUC4 is correlated with β-catenin in vivo, we examined Muc4 and β-catenin levels in colon tissue sections procured from three groups of mice: ApcMin mice that were treated with DSS (ApcMin/DSS), ApcMin mice overexpressing Claudin-1, which represents a more aggressive model of colorectal tumorigenesis, also treated with DSS (ApcMin/Cldn-1/ DSS) and lastly, ApcMin mice that were given water, representing a control group. This evidence concerns the gene CLDN1 and medical procedure.