FREM1 and metastatic neoplasm: Expression levels of miR-200a, miR-200b, miR-429, miR-30a-5p, miR-30a-3p, miR-30e, miR-30e-3p, CDH1, SLC22A24, C3orf52 and FREM1 were significantly decreased in metastatic (TNM stage III with pN+ and IV) compared to non-metastatic tumor (TNM stage I and II) (Fig. 2).