In general, EpCAM (epithelial cell adhesion molecule, BerEP4) (Fig 2B) and Calretinin (Fig 2C) were used as pan-epithelial and mesothelial markers, respectively, and if necessary, additional tumor specific markers were analyzed, such as TTF1 (thyroid transcription factor 1) (Fig 2E) in effusions originating from lung cancer patients. This evidence concerns the gene NKX2-1 and lung carcinoma.