We examined the effects of EWS shRNA plasmid transfection and TFL treatment on the status of methylation of DNA at the p53 promoter region and protein expression of p53, Puma, and Noxa that could promote apoptosis in both Ewing’s sarcoma SK-N-MC and RD-ES cell lines (Figure 6). This evidence concerns the gene ZC3H12D and Ewing sarcoma.