We examined the knockdown of EWS expression at mRNA and protein levels in human Ewing’s sarcoma SK-N-MC and RD-ES cell lines after scrambled shRNA plasmid transfection, EWS shRNA plasmid transfection, TFL treatment, and combination of EWS shRNA plasmid transfection and TFL treatment (Figure 3). Here, EWSR1 is linked to Ewing sarcoma.