Combination of EWS shRNA plasmid transfection and TFL treatment caused the highest increase in expression of pro-apoptotic Bax protein and decrease in expression of anti-apoptotic Bcl-2 protein, which could result in increase in the Bax: Bcl-2 ratio for increasing mitochondrial permeability in both Ewing’s sarcoma cell lines. This evidence concerns the gene BCL2 and Ewing sarcoma.