ZC3H12D and Ewing sarcoma: To reveal the molecular mechanisms for induction of both extrinsic and intrinsic pathways of apoptosis, we performed Western blotting with the protein samples from human Ewing’s sarcoma SK-N-MC and RD-ES cell lines following scrambled shRNA plasmid transfection, EWS shRNA plasmid transfection, TFL treatment, and combination of EWS shRNA plasmid transfection and TFL treatment (Figure 7).