We examined the knockdown of EWS expression at mRNA and protein levels in human Ewing’s sarcoma SK-N-MC and RD-ES cell lines after scrambled shRNA plasmid transfection, EWS shRNA plasmid transfection, TFL treatment, and combination of EWS shRNA plasmid transfection and TFL treatment (Figure 3). This evidence concerns the gene ZC3H12D and Ewing sarcoma.