EWSR1 and Ewing sarcoma: We performed Western blotting to examine the effects of scrambled shRNA plasmid transfection, EWS shRNA plasmid transfection, TFL treatment, and combination of EWS shRNA plasmid transfection and TFL treatment on the molecular events for induction of differentiation, inhibition of angiogenesis and invasion, and induction of apoptosis in human Ewing’s sarcoma SK-N-MC and RD-ES xenografts (Figure 8E).