Thereafter, we employed Western blotting to demonstrate that knockdown of EWS expression and TFL treatment most effectively down regulated the molecular markers of cell survival (p-Akt and NF-κB), angiogenesis (VEGF and b-FGF), and invasion (MMP-2 and MMP-9) in Ewing’s sarcoma SK-N-MC and RD-ES cells (Figure 5C). This evidence concerns the gene AKT1 and Ewing sarcoma.