We confirmed constitutive expression of EWS protein in Ewing’s sarcoma SK-N-MC and RD-ES cells and demonstrated modulation of molecular components for induction of differentiation and inhibition of angiogenesis and invasion leading to apoptosis in these malignant cells in culture and animal models after knockdown of EWS expression and TFL treatment. This evidence concerns the gene EWSR1 and Ewing sarcoma.