We used human Ewing’s sarcoma SK-N-MC and RD-ES cells for development of xenografts in nude mice, which were then subjected to scrambled shRNA plasmid transfection, EWS shRNA plasmid transfection, TFL treatment, and combination of EWS shRNA plasmid transfection and TFL treatment for assessment of alterations in tumor growth, histopathology, and molecular markers (Figure 8). The gene discussed is ZC3H12D; the disease is Ewing sarcoma.