We found that scrambled shRNA plasmid transfection group maintained characteristic tumor growth, EWS shRNA plasmid transfection or TFL treatment alone inhibited cell proliferation and induced cell death to some extent, but combination of EWS shRNA plasmid transfection and TFL treatment caused the most dramatic inhibition of cell proliferation and induction of massive cell death in both Ewing’s sarcoma xenograft models (Figure 8D). The gene discussed is ZC3H12D; the disease is Ewing sarcoma.