To visualize the differences in infection dynamics between the pneumolysin-deficient mutant and the wild-type strain, Tg(kdrl:mCherry)s896, zebrafish embryos were infected with the green fluorescent HlpA-GFP S. pneumoniae D39Δply strain and phagocytes were stained with anti-L-plastin. The gene discussed is LCP1; the disease is infection.