Similar results were obtained in TMX2-28 (estrogen-receptor-negative sub-line of the MCF-7 breast cancer cells [31]) stably overexpressing GFP, myc-PAK1 WT, or myc-PAK1 Y3F (Fig. 2d, anti-pY397-FAK and anti-FAK blots) indicating that this finding was not restricted to T47D cells. This evidence concerns the gene ESR1 and breast carcinoma.