They evaluated the phosphorylation/activation of HER-2, HER-3, EGFR (HERs) and downstream transducers in HER2-overexpressing colorectal and gastric cancer cell lines; in addition, the in vivo outcome of antibody-mediated HER-2 blockade with trastuzumab, reversible HER2-inhibition by lapatinib, and irreversible HER2-inhibition by afatinib inpatient-derived tumorgrafts and cell-line xenografts was also assessed. Here, ERBB2 is linked to gastric cancer.