Our studies show that long-term treatment (4 weeks) with media containing C-S and anti-androgens (Bic or MDV) (which mimics ADT in PC patients) increases expression of GRP, GRP-R and AKT2 (a downstream target gene of GRP/GRP-R signaling) [45, 46] in LNCaP PC cells (Figure 1A, 1B and 1C). This evidence concerns the gene GRPR and pachyonychia congenita.