To investigate the effect of Llgl1 expression on tumor forming capabilities in vivo, shControl, shLlgl1, shLlgl1 CD44hi/CD49flo, and shLlgl1 CD44lo/CD49fhi cells were injected in the mammary fat pads of NOD-SCID mice with matrigel into the mammary fat pads of NOD-SCID mice and evaluated for survival (Figure 7D). Here, LLGL1 is linked to neoplasm.