We elicited LCMV primed T cells in congenic wild type mice, purified CD4+ T cells on day 8 post-infection at the peak of T cell expansion, and adoptively transferred the cells into naïve Myd88-/-, IL-1R-/-, TLR2, 3, 4, 7, 9-/-, TLR3, 7, 9-/- and wild type mice. Here, TLR3 is linked to infection.