Confirming previous results [19], we show that ADAM10 and ADAM17 are expressed in GSCs (Fig. 1a) along with colocalisation of ADAM10 and ADAM17 and nestin [5]; moreover, we have previously reported increased mRNA levels in both glioblastoma tissues and corresponding cultured spheres established from some of the same patient samples used in the present study (including G002, G037, G049). Here, NES is linked to glioblastoma.