The antifungal protein PAF from Penicillium chrysogenum has been used to control disease by increasing cytosolic free Ca2+, which is involved in hyphal tip growth, hyphal branching, sporulation, spore germination, different infection structure formation, circadian clocks, and responses to environment stimuli including osmotic stress, heat shock, mechanical stimulations, and oxidative stresses. This evidence concerns the gene PCLAF and infection.