Subsequently, Kitamoto et al[13] performed another GWAS that containing 3 genes (PNPLA3, SAMM50, and PARVB) in the Japanese population, and the results showed that rs2896019 and rs3810622 in the PNPLA3 gene were associated with decreased serum TGs, increased AST and ALT in NAFLD patients, suggested that these 2 SNPs might play an important role in the development of NAFLD. The gene discussed is PNPLA3; the disease is metabolic dysfunction-associated steatotic liver disease.