Despite the physiological function of PNPLA3 has not been fully elucidated, it is generally regarded that this protein may be involved in the balance of lipid and the expression level regulated by energy intake.[24,25] Also, a number of genetic variants of PNPLA3 have been found a strong connection with NAFLD, especially the rs738409 locus. The gene discussed is PNPLA3; the disease is metabolic dysfunction-associated steatotic liver disease.