In a previous study, we had demonstrated that rs738409 loci in PNPLA3 gene was significantly associated with NAFLD (P = 0.0002).[15] As no study regarding the association of rs2896019 and rs3810622 and predisposition to NAFLD in other populations has yet been published, in this study, we will further evaluate the association of rs2896019 and rs3810622 polymorphisms in PNPLA3 gene with NAFLD based on a Han Chinese population, which represents the world's largest population, to further elucidate the genetic causes of NAFLD. Here, PNPLA3 is linked to metabolic dysfunction-associated steatotic liver disease.