Figure 3A indicates that the level of ROS in HIV-1 gp120 with cocaine is significantly increased (P < 0.0003) when compared to either cocaine (p < 0.01) or HIV-1gp120 (P < 0.003) alone. Figure. 3B shows AMPK is significantly increased in cocaine (p < 0.05), HIV-infection (p < 0.01) and cocaine with HIV infection (p < 0.001). Figure. 3C shows MAP/Tau is also significantly upregulated due to cocaine (p < 0.007), HIV-infection (p < 0.001) and cocaine with HIV infection (p < 0.005). Here, ITIH4 is linked to HIV infectious disease.