Lastly, we investigated whether the effect of six PD-linked missense parkin mutations (R33Q, R42P, G328E, R334C, T415N and C148R) located in nearby ISGylation sites (IBR domain) or autoinhibition-related regions (UBL domain and PUB motif) on parkin ISGylation (figure 10a,b). The gene discussed is PRKN; the disease is Parkinson disease.