GLI1 and lung adenocarcinoma: No available combination of antibodies allowed us to perform co-staining of Gli1 and E-Cadherin; however, we demonstrated that Gli1 and Gli2 expression correlated significantly in lung adenocarcinoma by IHC on FFPE samples of the Tianjin cohort (Figure 1B) and by RT-PCR on 63 freshly frozen samples of the UCSF cohort (Figure 1D).