Further, and owing to increased MGAT5 expression and GnT-V activity frequently observed in a cancer cell, E-cadherin is predominantly targeted by GnT-V-mediated glycosylation at the Golgi apparatus, where β1,6-GlcNAc-branched N-glycans are added to Asn-554 of E-cadherin. This evidence concerns the gene MGAT5 and cancer.