RELN and Alzheimer disease: In fact, a 5 min boiling of the sample prior to SDS-PAGE apparently diminished the Reelin in the AD cortex by up to 70% compared to that detected after 3 min at 98 °C, whereas the Reelin in the cortex from ND cases was less strongly affected by 5 min boiling, diminishing it only for ~25% with respect to 3 min denaturation.