Splenocytes were harvested from MC38 tumour-bearing mice treated as described in Fig. 6a,b on Day 19 (12 days after the first treatment) and stimulated with KSPWFTTL, the tumour associated antigen peptide presented by major histocompatibility complex class I (H-2Kb) for 48 h to detect antigen-specific CD8+ T cells. This evidence concerns the gene CD8A and neoplasm.