As chemotherapy and PDT of NCP@pyrolipid in combination with anti-PD-L1 caused effective regression of both primary tumours and distant tumours on syngeneic MC38 and CT26 mouse models, which we hypothesized was due to effective systemic antitumour immune responses, we investigated the antitumour immunity induced by chemotherapy/PDT of NCP@pyrolipid in combination with anti-PD-L1 in a syngeneic MC38 mouse model by enzyme-linked immunospot (ELISPOT) and flow cytometry. This evidence concerns the gene CD274 and neoplasm.