The Ttf-1-positive alveolar epithelium was dispersed in the nitrofen-induced CDH fetal lungs, in contrast to the controls that showed a well-structured Ttf-1-positive alveolar epithelium, whereas the ONO-1301SR-treated CDH lungs displayed preservation of the Ttf-1-positive alveolar epithelium compared to lungs without ONO-1301SR treatment (Fig 4A). This evidence concerns the gene TTF1 and congenital diaphragmatic hernia.