Pancreatic cancer cells apoptosis was increased and tumor growth was synergistically inhibited by thymoquinone combined with gemcitabine both in vitro and in vivo via modulating multiple molecular signaling targets, such as suppressing Notch1 and Notch intracellular domain (NICD), up-regulating PTEN (phosphatase and tensin homolog deleted on chromosome ten), and inactivating Akt/themammaliantargetofrapamycin (mTOR)/S6 signaling pathways. The gene discussed is AKT1; the disease is neoplasm.