Our analysis revealed that positive expression of CD133 was significantly associated with gross tumor morphology (P = 0.034), histologic type (P = 0.008), tumor invasion (pT) (P = 0.012), lymph node status (pN) (P < 0.001), sub-class of stage III (P < 0.001), but it was not associated with gender, age, tumor site, tumor size, surgery, intestinal obstruction, serum CEA, CA199 or CA242 levels (Table 1). This evidence concerns the gene PROM1 and intestinal obstruction.