DNMT3A mRNA levels were detected using semi-quantitative reverse-transcriptase PCR method in PBMCs from 29 healthy volunteers and 74 unselected de novo AML patient samples with known CR status, DNMT3A mutation (R882 mutation, n=9) and DNMT3A SNP status. The gene discussed is DNMT3A; the disease is acute myeloid leukemia.