To extend and validate the previous findings in another gene context, we chose a gastric cancer class II TSG REPRIMO. We designed three TALEs and a set of four gRNAs targeted to the proximal promoter of REPRIMO (Figure 7A) and we first validated these constructs in a low-expressing REPRIMO cell line, the triple negative breast SUM159 line (Figure 7B). Here, RPRM is linked to gastric cancer.