HTN1 and dental caries: In this study, we collected 16 stimulated whole saliva samples from 8 children with s-ECC before and after 4 weeks of treatment (equal amounts of the total protein were used for each sample) who were randomly chosen from the whole sample, to perform western blotting assay in order to learn the difference of protein expression of histatin-1 between different time points before and after treatment of dental caries.