Rspo3 is aberrantly expressed at high levels in approximately half of all Keap1-mutated human lung adenocarcinomas, and may promote cancer aggressiveness.45 In zebrafish, Rspo3 knockdown activates Wnt/β-catenin signaling, which is involved in anteroposterior patterning.46 A 2.61-fold downregulation in Rspo3 expression was observed in the RNAseq analysis on the RAG1−/− background, which was confirmed in quantitative RT–PCR in wild-type and Sgo1−/+ (Figure 4d). The gene discussed is KEAP1; the disease is cancer.