CDH1 and cancer: TGFβ is a critical regulator of EMT in multiple cancers through mediation of transcriptional repression of genes associated with the epithelial phenotype.3, 4 Treatment of PANC-1 cells with TGFβ over 48 h period induced ZEB1 and drastically reduced the amount of E-cadherin protein and mRNA in cells (Figures 5a and b, Mock), confirming that TGFβ-treated cells undergo EMT.