Consistent with the findings of colon cancer tissue microarray, we also found that miR-126 significantly suppressed the expression of CXCR4, the activity of RhoA, and the expression of RhoGEF, ROCK, PI3K, PAK, PKN and up-regulated the expression of ARHGAP5 in colon cancer cells. The gene discussed is ARHGAP5; the disease is colonic neoplasm.