The transfected glioma cells grown in differentiated (DMEM with 10% FBS allows to maintain mixture of cells with various lineage, Supplementary Figure S4) and stem cell conditions (NSA media supplemented with N2, B27, EGFFR and FGF) were stained using CD133, NESTIN, GFAP and OLIG2 markers. Here, NES is linked to central nervous system cancer.