Here, we established and characterized SNS-032-resistant sub-lines of the MYCN-amplified, ABCB1-negative neuroblastoma cell line UKF-NB-3 (UKF-NB-3rSNS-032300nM) and the non-MYCN-amplified, ABCB1-expressing neuroblastoma cell line SHEP (SHEPrSNS-0322000nM) to analyze the role of ABCB1 in neuroblastoma models of acquired SNS-032 resistance. This evidence concerns the gene ABCB1 and neuroblastoma.