ERBB2 and metastatic malignant neoplasm in the lymph nodes: Patient C was a 63-year-old female Asian diagnosed with gallbladder cancer with lymph node metastasis, which harbored HER2 gene amplification with a HER2/CEP17 ratio of 9.53 by FISH (Supplementary Figure S3) and was HER2 3+ by IHC.