The fluorescence signal of PTS1-tagged control GFPNb-V5 and SVVNb8-V5 adopts a dotted pattern in PC-3 prostate cancer cells, which colocalizes with the peroxisome markers PMP70 (Fig. 6a) and Pex14p (Fig. 6b). The gene discussed is ABCD3; the disease is Familial prostate cancer.