LiM6-TR and DLD1-TR are derivatives of colon cancer cell lines LS174T and DLD1 which are TRAIL resistant and have high cell surface and total Gal3 expression compared to their parental cell lines by flow cytometry, Western analysis, and confocal microscopy.10 Analogous to the flow cytometric analyses in Figure 1, we compared LiM6 to LiM6-TR and DLD1 to DLD1-TR, with respect to CSC (i.e., positivity for CD24, CD44, CD166, and EpCAM) and Gal3 positivity. This evidence concerns the gene CD44 and malignant colon neoplasm.