To ask whether AKTIP depletion results in a similar phenotype, we treated HPFs at different passages (p6 and p22) with shAKTIP (electronic supplementary material, figure S3a) and examined 7 days post-infection cells immunostained with the C20 antibody, which specifically reacts with prelamin A. shAKTIP HPFs at p22 displayed a higher frequency of prelamin A-positive cells than passage-matched control HPFs (figure 7a,b). This evidence concerns the gene AKTIP and infection.