LMNA and Hutchinson-Gilford progeria syndrome: In unextracted LV-progerin cells expressing both endogenous lamin A and progerin, and more effectively in unextracted HGPS cells, which are heterozygous for the LMNA mutation, the AKTIP signal in the cytoplasm was reduced and the nuclear rim staining was weaker and more discontinuous than in controls (figure 4a); the defective AKTIP localization at the nuclear rim of these cells was more evident in preparations extracted with Triton X-100 (figure 4b).