However, treatment of these co-cultures with LPS and IFN-γ together not only enhanced NO titer significantly but also rescued NO levels even in highest R: P ratio group (Fig. 3C) up to 96 h, demonstrating the significance of LPS and IFN concerted signaling in M1 retuning of tumor induced M2 phenotype in macrophages. The gene discussed is IFNG; the disease is neoplasm.