MAP3K13 and infection: To assess the efficiency of Cre-mediated LZK knockout, we infected primary CGNs isolated from homozygous LZKT/T mice on 2 DIV with AAV-GFP-Cre and observed a ~60% infection efficiency as early as three days post-infection in CGNs based on the percentage of GFP-positive cells in the total cell population (Fig. 5C).