KRAS and colorectal carcinoma: To determine if TIMP-1 could promote invasion in a KRAS-dependent manner in CRC cells, we stimulated the matched pair of isogenic DLD-1 cell clones (KRAS G13D and KRAS wt) with exogenously added recombinant TIMP-1 (rTIMP-1; 5 μg/mL) [40] and compared the invasive potential of these cells to that of a control group (no added rTIMP-1), in a Boyden chamber invasion assay.