TIMP1 and colorectal carcinoma: In order to further assess the biological effects of TIMP-1 on CRC cells, and its association to KRAS mutational status, we performed an anchorage-independent growth analysis of a matched pair of isogenic DLD-1 cell clones (KRAS G13D and KRAS wt), in which either the wild-type or mutant KRAS allele has been disrupted [38].