EGFP expression increased in the cancer cells that were cotransfected with EGFP-MBD1 and siUHRF1, compared with cancer cells transfected with EGFP-MBD1 and the normal control siRNA (siNC) overall and in individual cancer cells (Figure 3B, 3C), whereas expression of MBD1 itself did not change (Figure 3D). This evidence concerns the gene MBD1 and cancer.