To examine whether UHRF1 overexpression can influence LINE-1 methylation level (global DNA methylation level) in esophageal cancer cell lines, we transfected KYSE-30 cells, which exhibited low expression of UHRF1 and LINE-1 hypermethylation, with UHRF1 vector (Figure 2A) and analyzed LINE-1 methylation levels using a bisulfite PCR pyrosequencing assay. Here, UHRF1 is linked to esophageal cancer.