To determine whether ω-3 PUFAs inhibit proliferation and induce apoptosis of CRC cells are mediated by YAP, which directly promotes the expression of many proliferation and apoptosis related genes, including CTGF, AREG, EGR3, Cyr61, the IAP (inhibitor of apoptosis proteins) family members NAIP, BIRC5 and BIRC7, and the BCL2 family gene MCL1 [12, 18, 21], we infected the CRC cells by YAP (5SA), a constitutively active YAP mutant, to over-express YAP. This evidence concerns the gene AREG and colorectal carcinoma.