Stimulation of autophagy by 5-FU has previously been demonstrated in non-small cell lung cancer A549 cells [15], and was confirmed in the colon carcinoma HCT116 cell line by the detection of LC3 punctuation in ICC analysis, the formation of autophagosomes by transmission electron microscopy (TEM) and p62 degradation as well as LC3-I to II conversion in SDS-PAGE (Figure 1A and 1B, Figure 2A and 2C). The gene discussed is MAP1LC3A; the disease is colon carcinoma.