By 10 hours post-infection, MOMP and Hsp60 double-positive Chlamydia had increased to ~1.3μ in diameter (S1 Table) and ~80% of the cells contained 2–4 discrete regions of DNA and a subset of these regions of DNA density were juxtaposed to the MOMP-positive pole of the cell (Fig 1C). Here, HSPD1 is linked to infection.