EPCAM and cancer: Yet despite these lung cancer cells’ dynamic EpCAM expression, high capture yields were achieved when seeding the cells for 1 h in the microfluidic CTC capture chip with a nanoroughened glass surface (Rq = 150 nm) for both pre- and post-EMT A549 lung cancer cells, even at extremely low cancer cell concentrations (80 cells mL−1) (Fig. 2b & c).