Together, our results in Fig. 2 support that the RIE-generated nanorough glass surfaces can achieve efficient capture of CTCs independently of the cancer cell’s epithelial or mesenchymal state or EpCAM expression, demonstrating the applicability of the microfluidic CTC capture device for the capture and enumeration of rare tumor cells from heterogeneous cell samples and throughout a tumor’s metastatic progression, even in the setting of a dynamic EMT process. This evidence concerns the gene EPCAM and neoplasm.