Evidence that DVL1 can be specifically localized to the nucleolus was further shown by ectopic expression of FLAG-tagged DVL1 in fibroblasts lacking endogenous DVL1 protein (S3b Fig) as well as by immuno-electron microscopy of MCF7 cells stably expressing Wnt5a, and of MDA-MB-231 breast cancer cells (S3c Fig). This evidence concerns the gene DVL1 and breast cancer.