TGFβ ligands are also expressed in the region at the onset of HE formation: tgfb1a and tgfb1b are expressed in the endothelium, including the DA at 15 hpf, 24 hpf, and 27 hpf (Figures 1B, 1C, S1B, and S1C). The gene discussed is TGFB1; the disease is hereditary elliptocytosis.