In order to further explore the composition of these complexes, affinity purification of E-cadherin/ΔCDCP1 complexes employed T47D and MDA-MB-231 breast cancer cells stably expressing an E-cadherin-glutathione S-transferase (GST) fusion protein and an adenoviral vector encoding FLAG-tagged ΔCDCP1. The gene discussed is CDH1; the disease is breast cancer.