We then established miR-146a-5p stably overexpressing H1299 and SPCA-1 cell lines in order to analyze the biological functions of miR-146a-5p, as well as the post-transcriptional regulatory relationship between miR- 146a- 5p and CCND1 and CCND2 in NSCLC cells. This evidence concerns the gene CCND1 and non-small cell lung carcinoma.