To assay TKI-induced anti-tumor responses we used EGFRL858R and the EGFR downstream pathway proteins, ERK1/2 (phospho-ERK1/2 Thr 202/Tyr 204), AKT (phospho-AKT Thr 308) and a signal transducer and activator of transcription 3 (phospho-STAT3 Tyr 705) [6, 20]. This evidence concerns the gene STAT3 and neoplasm.